METHOD FOR REPLICATING HEPATITIS C VIRAL GENOME

Title:

METHOD FOR REPLICATING HEPATITIS C VIRAL GENOME

Document Type and Number:

Japanese Patent JPH05344889

Kind Code:

A

Abstract:

PURPOSE: To efficiently replicate a hepatitis C virus(HCV) genome by the in vitro cell culture.

CONSTITUTION: This method for replicating a hepatitis C virus genome is characterized by infecting an animal cell infected with a murine retrovirus with a hepatitis C virus and culturing the cell. The animal cell used is preferably human-T cell and more preferably Molt-4 cell or HPB cell. The murine retrovirus is preferably amphotropic murine leukemic cell virus and present as a complex with a sarcoma virus in the animal cell. One of the preferred cells is Molt-4 cell containing a sarcoma virus-amphotropic murine leukemic virus complex and this is called Molt-4Ma. The Molt-4Ma cell is obtained by co-cultivating the Molt-4 cell with StL-mink cell infected with amphotropic murine leukemic virus.

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Inventors:

YOSHIKURA HIROSHI
SHIMIZU YOKO
IWAMOTO AIKICHI
HIJIKATA MINAKO
ROBAATO EICHI PAASERU

Application Number:

JP15378692A

Publication Date:

December 27, 1993

Filing Date:

June 12, 1992

Export Citation:

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Assignee:

YOSHIKURA HIROSHI
SHIMIZU YOHKO

International Classes:

C12N5/10; C12N7/00; C12N15/00; C12N15/09; C12N15/51; C12Q1/70; C12R1/92; (IPC1-7): C12N15/51; C12N5/10; C12N7/00; C12Q1/70

Attorney, Agent or Firm:

Aoki Akira (4 outside)

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