Title:
METHOD FOR SCREENING GOOD SPERM
Document Type and Number:
Japanese Patent JP2974525
Kind Code:
B2
Abstract:
PURPOSE: To screen the good sperm by passing a sperm suspension through a PSA-affinitive glass bead column container and allow glass beads to adsorb the sperm having intact acrosome thereon.
CONSTITUTION: Glass wool is spread on the bottom of a vertical container, and washed glass beans are charged in the container. A phosphoric acid buffer solution containing PSA in a concentration of 0.1-1mg/ml is filled in the container and subsequently allowed to stand at 4°C for 3-21 days. A sperm suspension is passed through the obtained PSA-affinitive glass bead column container, and allowed to stand for approximately 15min at room temperature to adsorb sperms having intact acrosome. Non-adsorbed sperms are eluted with a phosphoric acid buffer solution, and subsequently a phosphoric acid buffer solution containing mannose is poured to the container at room temperature for 10min to release the adsorbed sperms for their recovery.
Inventors:
JINNO MASAO
Application Number:
JP33298292A
Publication Date:
November 10, 1999
Filing Date:
December 14, 1992
Export Citation:
Assignee:
JINNO MASAO
International Classes:
A01K67/02; A61B17/425; A61B17/43; A61K35/52; B01D15/00; G01N33/50; (IPC1-7): G01N33/50; A01K67/02; A61B17/43; A61K35/52; B01D15/00
Attorney, Agent or Firm:
Akihide Sugimura (5 outside)