Title:
METHOD FOR STABILIZING L-METHIONINE-GAMMA-LYASE
Document Type and Number:
Japanese Patent JP3125610
Kind Code:
B2
Abstract:
PURPOSE: To simply stabilize L-methionine-γ-lyase for determination, etc., of amino acid for a long period by lyophilizing a solution containing L- methionine-γ-lyase, an oligosaccharide, a water-soluble polysaccharide, a sugar alcohol, a protein, an acidic amino acid, etc.
CONSTITUTION: A solution containing L-methionine-γ-lyase and one or more kinds of substances selected from an oligosaccharide such as saccharose, maltose, lactose, trehalose, raffinose or maltotriose, a water-soluble polysaccharide such as dextran, a sugar alcohol such as sorbitol, mannitol or xylitol, a protein such as hydrolyzate of gelatin, an acidic amino acid such as glutamin acid, aspartie acid or an alkali metal salt thereof, glutathione, N-acetylcysteine is prepared and the solution is lyophilized to simply stabilize L-methionine-γ-lyase useful for detection and determination of amino acid such as L-methionine, L-cysteine so as to be able to stably store for a long period.
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Inventors:
Koichi Nakao
Ken Kazuno
Tsuda
Ken Kazuno
Tsuda
Application Number:
JP33728094A
Publication Date:
January 22, 2001
Filing Date:
December 26, 1994
Export Citation:
Assignee:
Wako Pure Chemical Industries, Ltd.
International Classes:
C12N9/88; C12N9/96; C12R1/38; (IPC1-7): C12N9/96; C12N9/88
Domestic Patent References:
| JP62221697A | ||||
| JP57118790A | ||||
| JP223866A | ||||
| JP62130687A |
Other References:
Ａｎａｌｙｔｉｃａｌ Ｂｉｏｃｈｅｍｉｓｔｒｙ，Ｖｏｌ．１３８，（１９８４），ｐ．４２１−４２４