To provide the concrete uses of a nano-composite enzyme obtained by immobilizing an enzyme and a hydrophilic polymer chain on a water-insoluble substrate (especially in a fine particle form) in various industrial fields.

This method for using the enzyme compound comprises bringing a substrate selected from the following group B into contact with the enzyme composite whose constituting enzyme is one or more enzymes selected from the following group A. The group A: protease, lipase, cellulase, maltase, saccharase, amylase, chitinase, cytochromes, lactase, and dextranase. The group B: medicinal raw materials, substances to be labeled with labeling enzymes, skin surface substances, food precursors, environmental loading substances, substances to be washed, and fibers to be treated with enzymes.