核酸および/またはポリペプチドを検出するための方法および装置

Japanese Patent JP2007534961

A

A method for the detection and/or quantification of at least one target nucleic acid or target polypeptide in a sample of nucleic acids or polypeptides comprising the steps of: a) providing a sample comprising nucleic acids or polypeptides; b) labeling the nucleic acids or polypeptides with a ligand conjugate, the ligand conjugate comprising a first element binding to the nucleic acids or polypeptides and a second element which is a capture ligand; c) contacting the nucleic acid-ligand conjugates or polypeptide-ligand conjugates with at least one capture probe, the capture probe hybridizing with or binding to at least one target nucleic acid or target polypeptide; d) adding i) an oxidoreductase enzyme, wherein the oxidoreductase enzyme is recognized by the capture ligand, or ii) a complex comprising an oxidoreductase enzyme bound to a capture receptor, the capture receptor capable of binding to the capture ligand; e) adding a redox polymer, the redox polymer binding to the oxidoreductase enzyme, thereby resulting in the transfer of electrons from the enzyme via the redox polymer to an electrode surface; and f) detecting and/or quantifying the presence of the target nucleic acid(s) or target polypeptide(s).

Gao, Jician
Xie, Hong
You, Yuan Hong

JP2007510663A

November 29, 2007

April 29, 2004

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Agency for Science, Technology and Research

G01N27/416; C12Q1/00; C12Q1/26; C12Q1/68; G01N27/327; G01N33/53; G01N33/543; G01N33/553; G01N37/00

JP2001286298A	2001-10-16
JP2003164293A	2003-06-10
JP2003514924A	2003-04-22
JP2003517419A	2003-05-27
JP2003524168A	2003-08-12
JP2001286298A	2001-10-16
JP2003164293A	2003-06-10
JP2003514924A	2003-04-22
JP2003517419A	2003-05-27
JP2003524168A	2003-08-12

Shoichi Okuyama
Arihara Koichi
Matsushima Tetsuo

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