Title:
ヌクレオチドを標識付けする方法、標識ヌクレオチド及び有用な中間体
Document Type and Number:
Japanese Patent JP4981197
Kind Code:
B2
Abstract:
The present invention provides improved methods for labeling nucleotides. The method of the invention for labeling nucleotides comprises the steps of: reacting a reactive moiety of a linker, which linker is a platinum compound having a stabilizing bridge and two reactive moieties, with an electron donating moiety of a spacer, which spacer comprises a chain having at least four atoms and at least one heteroatom in the chain, which spacer further comprises said electron donating moiety at one end of the chain and a reactive moiety at the other end of the chain; reacting the reactive moiety of said spacer with a label; reacting the other reactive moiety of said linker with a nucleotide. A major advantage of the invention is that all nucleotides can be labeled by the method of the invention, whereas until now the attachment of a label was mostly restricted to one or certain nucleotides.
Inventors:
Houthot, Hendrick Yang
Reedy, Yang
Yersuma, Theinka
Heath Bry, Rob Yot.
Volkers, Hermann Har.
Reedy, Yang
Yersuma, Theinka
Heath Bry, Rob Yot.
Volkers, Hermann Har.
Application Number:
JP51741498A
Publication Date:
July 18, 2012
Filing Date:
October 08, 1997
Export Citation:
Assignee:
Claretech Biotechnology B.Far.
International Classes:
C07H23/00; C07F15/00; C12N15/09; C07H21/00; C12Q1/68
Domestic Patent References:
| JP5508853A | ||||
| JP7507576A | ||||
| JP884600A | ||||
| JP698797A | ||||
| JP61164160A | ||||
| JP7184696A | ||||
| JP7507804A | ||||
| JP11505533A | ||||
| JP2010166914A |
Attorney, Agent or Firm:
Keiichiro Saikyo