Title:
マデカソサイドおよびターミノロサイドに富むセンテラアシアチカ抽出物を調製するための方法
Document Type and Number:
Japanese Patent JP4643275
Kind Code:
B2
Abstract:
Production of an extract (A) of Centella asiatica, containing a mixture of madecassoside (I) and terminoloside (II), involves: (a) extracting above-ground plant parts with alcoholic solvent; (b) passing the extract through anionic resin; (c) selectively defatting the eluate; (d) concentrating the aqueous alcoholic phase to an aqueous phase, then filtering; (e) passing the aqueous phase through cationic resin then anionic resin; (f) stabilizing and (g) chromatographing. Production of an extract (A) of Centella asiatica, containing a mixture of madecassoside (I) and terminoloside (II), involves: (a) extracting above-ground plant parts with alcoholic solvent; (b) passing the extract through anionic resin; (c) selectively defatting the eluate by liquid-liquid extraction; (d) concentrating the aqueous alcoholic phase to an aqueous phase, then filtering; (e) passing the aqueous phase through cationic resin then anionic resin; (f) stabilizing by addition of alcohol; (g) selectively chromatographing, and (h) recovering the (I)/(II) mixture. An Independent claim is included for the extracts (A) obtained by the process, containing more than 95 wt. % of the (I)/(II) mixture and preferably having an overall (I) content of 30-70%.
Inventors:
Ruwaso Allen
Scene gerrard
Theron Eric
Scene gerrard
Theron Eric
Application Number:
JP2004566117A
Publication Date:
March 02, 2011
Filing Date:
December 10, 2003
Export Citation:
Assignee:
F. Hoffman-La Roche Age
International Classes:
C07J63/00; A61K8/63; A61K8/97; A61K31/56; A61K31/704; A61K36/00; A61K36/23; A61P1/04; A61P9/00; A61P9/14; A61P17/00; A61P17/04; A61P17/06; A61P17/16; A61P19/00; A61P19/02; A61P29/00; A61P37/00; A61P37/08; A61P39/00; A61P43/00; A61Q17/00; A61Q19/00; A61Q19/08; B01D11/02; B01J20/281; B01J39/04; B01J41/04; C02F1/42
Domestic Patent References:
| JP10279590A |
Foreign References:
| GB1123288A |
Other References:
BRINKHAUS B,PHYTOMEDICINE,ドイツ,GUSTAV FISCHER VERLAG,2000年10月,V7 N5,P427-448
Chem. Pharm. Bull.,2001年,Vol.49, No.10,p.1368-1371
Chem. Pharm. Bull.,2001年,Vol.49, No.10,p.1368-1371
Attorney, Agent or Firm:
Hatsushi Shimizu
Koichi Niimi
Koichi Niimi