Ｂ細胞悪性腫瘍及び他のがんの治療に有用な自己Ｔ細胞を産生する方法、並びにその組成物

Japanese Patent JP7087011

B2

Provided herein are methods for manufacturing T cells. Incertain embodiments, methods for manufacturing T cells which express a cell surface receptor that recognizes a specific antigenic moiety on the surface of a target cell are provided. Such methods may include, but are not limited to, Steps of (1 ) enriching a population of lymphocytes obtained from a donor subject; (2) stimulating the population of lymphocytes with one or more T- cell stimulating agents to produce a population of activated T cells, wherein the Stimulation is performed in a closed System using serum-free culture medium; (3) transducing the population of activated T cells with a viral vector comprising a nucleic acid molecule which encodes the cell surface receptor, using a single cycle transduction to produce a population of transduced Tcells, wherein the transduction is performed in a closed System using serum-free culture medium; and (4) expanding the population of transduced Tcells for a predetermined time to produce a population of engineered T cells, wherein the expansion is performed in a closed System using serum-free culture medium. Also provided herein are populations of engineered T cells produced by the methods described herein and pharmaceutical compositions thereof.

Better mark
Feldman Stephen A.
Rosenberg Stephen A.

JP2020007980A

June 20, 2022

January 22, 2020

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Kite Pharma, Inc

C12N5/10; A61K35/17; A61P35/00; A61P35/02; C12N5/0783; C12N15/12; C12N15/867

WO2009119793A1

Cancer Gene Therapy,2002年,Vol.9,p.613-623
Cytotherapy,2013年,Vol.15,p.1406-1415
Cytotherapy,2008年,Vol.10, No.4,p.406-416

Patent Business Corporation Saegusa International Patent Office

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