Title:
核酸リガンドによる標的結合を検出する方法および試薬
Document Type and Number:
Japanese Patent JP2005517456
Kind Code:
A
Abstract:
The present invention provides novel methods and reagents for detecting the binding of protein targets to nucleic acid ligands. Using Universal Protein Stains (UPS), proteins bound by nucleic acid ligands may be labeled with a detectable moiety. The methods and reagents are particularly useful for the detection of protein targets bound to multiplexed arrays of nucleic acid ligands. The present invention also provides novel methods for the multiplexed evaluation of photocrosslinking nucleic acid ligands. The methods allow one simultaneously to: (1) evaluate the performance (dynamic range) of a plurality of photocrosslinking nucleic acid ligands; and (2) assess the specificity of each photocrosslinking nucleic acid ligand for its cognate target protein. Photocrosslinking nucleic acid ligands with the most desirable properties can then be selected for use in diagnostic and prognostic medical assays. The present invention also provides a photocrosslinking nucleic acid ligand that binds specifically to HIV gp120MN.
Inventors:
Gold, rally
Switch, Dominique A
Smith, Jonathan Drew
Schneider, Daniel Jay
Grief, chad
Switch, Dominique A
Smith, Jonathan Drew
Schneider, Daniel Jay
Grief, chad
Application Number:
JP2003569875A
Publication Date:
June 16, 2005
Filing Date:
February 10, 2003
Export Citation:
Assignee:
SomaLogic, Inc.
International Classes:
C12M1/00; C12M1/34; C12N15/09; C12N15/115; C12Q1/25; C12Q1/28; C12Q1/42; C12Q1/68; G01N21/78; G01N33/543; G01N33/566; G01N33/53; G01N33/58; G01N33/68; A61K38/00; (IPC1-7): C12N15/09; C12Q1/25; C12Q1/28; C12Q1/42; C12Q1/68; G01N21/78; G01N33/53; G01N33/543; G01N33/566
Attorney, Agent or Firm:
Kazuo Shamoto
Tadashi Masui
Yasushi Kobayashi
Akio Chiba
Hiroyuki Tomita
Hiroko Ejiri
Tadashi Masui
Yasushi Kobayashi
Akio Chiba
Hiroyuki Tomita
Hiroko Ejiri