Title:
細胞増殖方法ならびに組織の修復および再生のための医薬
Document Type and Number:
Japanese Patent JP4936341
Kind Code:
B2
Abstract:
The present invention relates to a method for growing, rapidly and massively ex vivo, cells collected from a living subject to provide a safe and effective pharmaceutical preparation for biological tissue repair/regeneration. Specifically, the present invention relates to a method for growing cells in a sample collected from a living subject by culturing the cells in a medium containing allogeneic (including autogenic) serum. Preferably the allogeneic serum has been determined as being negative for a serum tumor marker and/or an infectious factors, and the amount of the anticoagulant (e.g., heparin, a heparin derivative, or a salt thereof) added to the collected sample is less than 5 U/mL with respect to the volume of the sample or the amount of the anticoagulant in the medium at the start of culture is less than 0.5 U/mL. The present invention further relates to use of the method.
Inventors:
Osamu Osamu
Takahiro Kiyohiro
Takahiro Kiyohiro
Application Number:
JP2009532060A
Publication Date:
May 23, 2012
Filing Date:
September 10, 2008
Export Citation:
Assignee:
Hokkaido Public University Corporation Sapporo Medical University
International Classes:
C12N5/0775; A61K35/12; A61K35/15; A61K35/16; A61K35/28; A61P1/16; A61P1/18; A61P3/00; A61P3/06; A61P3/08; A61P3/10; A61P9/00; A61P9/10; A61P13/08; A61P13/12; A61P25/00; A61P25/28; A61P43/00; C12N5/07; C12N5/074; C12N5/0797
Domestic Patent References:
| JP2005531322A | 2005-10-20 | |||
| JP2006055106A | 2006-03-02 | |||
| JP2006034118A | 2006-02-09 | |||
| JP2006136281A | 2006-06-01 |
Attorney, Agent or Firm:
Seiji Ohno
Koji Morita
Reiko Tanaka
Yuko Matsuniya
Ken Kitano
Natsuki Ito
Koji Morita
Reiko Tanaka
Yuko Matsuniya
Ken Kitano
Natsuki Ito