Title:
METHODS AND COMPOSITIONS FOR INCREASED DOUBLE STRANDED RNA PRODUCTION
Document Type and Number:
Japanese Patent JP2022087312
Kind Code:
A
Abstract:
To provide methods and compositions for increased double stranded RNA production.SOLUTION: Methods and materials for improved in vivo production of dsRNA are presented. Yields of dsRNA are significantly increased in the presence of capsid protein. The improved yield of dsRNA is not dependent on the presence of specific cognate binding sites for capsid protein associated with the dsRNA, but is dependent on capsid protein. For example, presented are methods for producing unencapsidated dsRNA in a microbial cell, wherein the dsRNA is co-expressed with a coat protein gene encoding a capsid protein.SELECTED DRAWING: None
Inventors:
JOHN L KILLMER
PATRICK D MCLAUGHLIN
JUAN PEDRO HUMBERTO ARHANCET
PATRICK D MCLAUGHLIN
JUAN PEDRO HUMBERTO ARHANCET
Application Number:
JP2022067530A
Publication Date:
June 09, 2022
Filing Date:
April 15, 2022
Export Citation:
Assignee:
APSE INC
International Classes:
C12N15/113; C07K14/08; C12N15/10; C12N15/11; C12N15/33; C12N15/40; C12N15/63; C12N15/71; C12N15/77; C12N15/81; C12N15/90; C12P19/34
Attorney, Agent or Firm:
Shusaku Yamamoto
Morishita Natsuki
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto
Morishita Natsuki
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto
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