Title:
ポリメラーゼＩＩ（Ｐｏｌ－ＩＩ）ベースのガイドＲＮＡ発現のための方法および組成物
Document Type and Number:
Japanese Patent JP7160891
Kind Code:
B2
Abstract:
Compositions and methods are provided for editing nucleotides and/or altering target sites in the genome of a cell. The methods and compositions employ a recombinant DNA construct comprising a Pol-II promoter operably linked to a polynucleotide encoding a single guide RNA, wherein said guide RNA is capable of forming a guide RNA/Cas endonuclease complex, wherein said complex can bind to and cleave a target site sequence in the genome of a cell such as a eukaryote cell. The present disclosure further describes methods and compositions employing said recombinant DNA construct to modify the genome of non-conventional yeast.
Inventors:
Bendez, Felipe Oceas
Fan, Xiao Chun
Frisch, Ryan El
Hong, Sun-Pyo
Fan, Xiao Chun
Frisch, Ryan El
Hong, Sun-Pyo
Application Number:
JP2020219041A
Publication Date:
October 25, 2022
Filing Date:
December 28, 2020
Export Citation:
Assignee:
Danisco US Inc.
International Classes:
C12N15/09; C12N1/19; C12N15/11; C12N15/79; C12N15/81
Foreign References:
| WO2015004241A2 | ||||
| WO2015006747A2 | ||||
| WO2015138855A1 |
Other References:
YANGBIN GAO; ET AL,SELF-PROCESSING OF RIBOZYME-FLANKED RNAS INTO GUIDE RNAS IN VITRO AND IN VIVO FOR CRISPR-MEDIATED GENOME EDITING.,JOURNAL OF INTEGRATIVE PLANT BIOLOGY,BLACKWELL PUBLISHING,2014年04月06日,VOL:56, NR:4,PAGE(S):343 - 349,http://dx.doi.org/10.1111/jipb.12152
Protein Cell, 2015, Vol.6, No.9, pp.689-692
Molecular Cell, 2014, Vol.54, pp.689-710
Scientific Reports, 16 Dec. 2015, Vol.5, Article No.18341, pp.1-8
Protein Cell, 2015, Vol.6, No.9, pp.689-692
Molecular Cell, 2014, Vol.54, pp.689-710
Scientific Reports, 16 Dec. 2015, Vol.5, Article No.18341, pp.1-8
Attorney, Agent or Firm:
Yamazaki Yuzo
Toshiaki Akamatsu
Tadao Naito
Chihiro Imai
Park Shinon
Toshiaki Akamatsu
Tadao Naito
Chihiro Imai
Park Shinon