Title:
METHODS AND COMPOSITIONS FOR TARGETED SINGLE-STRANDED CLEAVAGE AND TARGETED INTEGRATION
Document Type and Number:
Japanese Patent JP2016040252
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide methods and composition that generate single-stranded breaks (nicks) in a double-stranded DNA and facilitate targeted integration by homologous recombination at the nicked site, without simultaneously generating error-prone NHEJ repair in mammalian/human cells.SOLUTION: A protein complex comprises: a fusion protein comprising a DNA-binding domain and at least one catalytically inactive cleavage domain or cleavage half-domain; and a second fusion protein comprising a zinc finger domain and a catalytically active cleavage half domain. The catalytically inactive cleavage half domain forms a heterodimer with the catalytically active cleavage half-domain of the second fusion protein.SELECTED DRAWING: None
Inventors:
WANG JIANBIN
Application Number:
JP2015176328A
Publication Date:
March 24, 2016
Filing Date:
September 08, 2015
Export Citation:
Assignee:
SANAGAMO BIOSCIENCES INC
International Classes:
C07K19/00; C07K14/47; C12N9/22; C12N15/09
Foreign References:
| WO2007139898A2 | 2007-12-06 |
Other References:
JPN6017007264; Ｃｅｌｌ （２００４） ｖｏｌ．１１７， ｉｓｓｕｅ ２， ｐ．１７１-１８４
Attorney, Agent or Firm:
Atsushi Aoki
Takashi Ishida
Fukumoto product
Tetsuji Koga
Yoichi Watanabe
Ryotaro Takei
Kazumi Nakamura
Takashi Ishida
Fukumoto product
Tetsuji Koga
Yoichi Watanabe
Ryotaro Takei
Kazumi Nakamura