Title:
標的核酸を検出する方法
Document Type and Number:
Japanese Patent JP7245126
Kind Code:
B2
Abstract:
In one embodiment, the present invention is a nucleic acid probe for detecting a target nucleic acid. At least one terminal of the probe-binding region in the target nucleic acid is a guanine base, and one or more cytosine bases are present within 1 to 7 bases from the guanine base in the probe-binding region. The nucleic acid probe hybridizes with the probe-binding region of the target nucleic acid. The nucleic acid probe comprises an oligonucleotide having a cytosine base facing the guanine base on a terminal and a fluorescent dye conjugated to the cytosine base. The fluorescent dye is a fluorescent dye that is quenched by the interaction with a guanine base. The oligonucleotide is completely complementary to the nucleic acid in the probe-binding region except the one or more cytosine bases present within 1 to 7 bases from the terminal guanine base. The base in the oligonucleotide facing the cytosine base closest to the terminal guanine base among the one or more cytosine bases is a base having no fluorescence-quenching effect.
Inventors:
Ryohei Saeki
Application Number:
JP2019119734A
Publication Date:
March 23, 2023
Filing Date:
June 27, 2019
Export Citation:
Assignee:
Eiken Chemical Co., Ltd.
International Classes:
C12Q1/6825; C12Q1/6827; G01N33/53; G01N33/536
Domestic Patent References:
| JP2008199965A | ||||
| JP201390622A | ||||
| JP2010273660A | ||||
| JP2015128400A | ||||
| JP201234688A | ||||
| JP2002275A |
Foreign References:
| WO2014157377A1 |
Other References:
Nucleic Acids Research,2001年,vol.29,No.6,e34,p.1-5
Attorney, Agent or Firm:
Yoshiki Hasegawa
Yoshinori Shimizu
Katsusuke Kimoto
Hiroshi Abe
Akiteru Tamura
Yoshinori Shimizu
Katsusuke Kimoto
Hiroshi Abe
Akiteru Tamura
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