ゲノム編集のための方法

Japanese Patent JP7184364

B2

The present invention provides a method of producing a cell having a scarless genome sequence wherein an exogenous nucleic acid sequence inserted into a targeted region in the genome is completely excised, wherein the exogenous nucleic acid sequence comprises a nucleic acid sequence homologous to a genome sequence in the targeted region at each end and one or more sequence-specific nuclease-recognizing site(s) between the two homologous nucleic acid sequences, and wherein the method comprises: (1) introducing the sequence-specific nuclease or a nucleic acid encoding the same into a host cell having a genome sequence into which the exogenous nucleic acid sequence is inserted; and (2) culturing the cell obtained in step (1), thereby causing double-strand break at the sequence-specific nuclease-recognizing site(s) and the subsequent microhomology-mediated end joining or single-strand annealing between the resulting broken ends that contain the homologous nucleic acid sequences to generate a cell having a scarlessly reverted genome sequence in which the exogenous nucleic acid sequence is completely excised from the targeted region.

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Walzen, Knut
Kim, Shinil
Tomoko Matsumoto

JP2019505389A

December 06, 2022

August 02, 2017

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Kyoto University

C12N15/09; C12N5/10; C12N15/55; C12N15/62

JP2015177788A
JP2013537799A

WO2015088643A1

Mutat Res.,2016年01月02日,Vol.788,pp.17-24

Takashima Hajime
Mitsuyoshi Kamada
Kyoko Doi
Yaeko Tamura
Hirofumi Taima
Atsuko Akai
Tomiya Tosaki

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