Title:
METHODS OF MEASURING ADAMTS13-MEDIATED IN VIVO CLEAVAGE OF VON WILLEBRAND FACTOR AND USES THEREOF
Document Type and Number:
Japanese Patent JP2015213513
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide methods of measuring ADAMTS13-mediated in vivo cleavage of circulating von Willebrand factor (VWF) that mediates primary hemostasis supporting the adhesion of platelets to damaged blood vessels, and the use of the methods.SOLUTION: Methods of detecting VWF fragments in the blood of a subject show the in vivo activity of ADAMTS13 by detection (i.e., visualization and even quantification) of the amount of circulating fragments of cleaved VWF. The methods are based on SDS-PAGE combined with immunoblotting using specific antibodies against VWF. The VWF antibodies are specific for different fragments of VWF.
Inventors:
KATALIN VARADI
HANSPETER ROTTENSTEINER
PETER TURECEK
HANS-PETER SCHWARZ
JUTTA SCHREINER
HANSPETER ROTTENSTEINER
PETER TURECEK
HANS-PETER SCHWARZ
JUTTA SCHREINER
Application Number:
JP2015134417A
Publication Date:
December 03, 2015
Filing Date:
July 03, 2015
Export Citation:
Assignee:
BAXTER INT
BAXTER HEALTHCARE SA
BAXTER HEALTHCARE SA
International Classes:
C12Q1/37; G01N33/53
Domestic Patent References:
| JP2007530912A | 2007-11-01 | |||
| JP2005535315A | 2005-11-24 |
Foreign References:
| WO2006085441A1 | 2006-08-17 | |||
| WO2004035778A1 | 2004-04-29 |
Other References:
JPN6014018161; ＲＥＩＴＥＲ， Ｒ．Ａ． ｅｔ ａｌ．: '"Ｃｈａｎｇｅｓ ｉｎ ｖｏｎ Ｗｉｌｌｅｂｒａｎｄ ｆａｃｔｏｒ-ｃｌｅａｖｉｎｇ ｐｒｏｔｅａｓｅ （ＡＤＡＭＴＳ１３） ａｃｔｉｖｉｔｙ ａｆｔｅｒ ｉｎｆｕｓｉｏｎ ｏｆ ｄｅｓｍｏｐｒｅｓｓ' ＢＬＯＯＤ Ｖｏｌ．１０１， Ｎｏ．３, 20030201, Ｐ．９４６-９４８
JPN6014018164; ＦＵＲＬＡＮ， Ｍ． ｅｔ ａｌ．: '"ｖｏｎ Ｗｉｌｌｅｂｒａｎｄ ｆａｃｔｏｒ-ｃｌｅａｖｉｎｇ ｐｒｏｔｅａｓｅ ｉｎ ｔｈｒｏｍｂｏｔｉｃ ｔｈｒｏｍｂｏｃｙｔｏｐｅｎｉｃ ｐｕｒｐｕｒａ ａｎｄ ｔｈｅ ｈｅｍｏｌｙｔｉｃ-ｕｒ' ＴＨＥ ＮＥＷ ＥＮＧＬＡＮＤ ＪＯＵＲＮＡＬ ＯＦ ＭＥＤＩＣＩＮＥ Ｖｏｌ．３３９， Ｎｏ．２２, 19981126, Ｐ．１５７８-１５８４
JPN6014018162; 日裏久英: '「ＴＭＡの診断と血小板輸血の指標に重要なＡＤＡＭＴＳ１３活性の新規測定法の開発」' 臨床病理 Ｖｏｌ．５５， Ｎｏ．１, 200701, Ｐ．６９-７５
JPN6015008544; ＴＳＡＩ， Ｈ．Ｍ． ｅｔ ａｌ．: '"Ｐｈｙｓｉｏｌｏｇｉｃ ｃｌｅａｖａｇｅ ｏｆ ｖｏｎ Ｗｉｌｌｅｂｒａｎｄ ｆａｃｔｏｒ ｂｙ ａ ｐｌａｓｍａ ｐｒｏｔｅａｓｅ ｉｓ ｄｅｐｅｎｄｅｎｔ ｏｎ ｉｔｓ ｃｏｎｆｏｒｍａｔｉｏｎ' ＢＬＯＯＤ Ｖｏｌ．８７， Ｎｏ．１０, 19960515, Ｐ．４２３５-４２４４
JPN6015037768; ＦＩＳＣＨＥＲ， Ｂ．Ｅ． ｅｔ ａｌ．: '"Ｓｔｒｕｃｔｕｒａｌ ａｎａｌｙｓｉｓ ｏｆ ｒｅｃｏｍｂｉｎａｎｔ ｖｏｎ Ｗｉｌｌｅｂｒａｎｄ ｆａｃｔｏｒ ｐｒｏｄｕｃｅｄ ａｔ ｉｎｄｕｓｔｒｉａｌ ｓｃａｌｅ ｆｅｒｍｅｎｔａｔｉｏｎ' ＦＥＢＳ ＬＥＴＴ． Ｖｏｌ．３７５， Ｎｏ．３, 19951120, ｐｐ．２５９-２６２
JPN6015037770; ＳＣＨＬＯＫＡＴ， Ｕ． ｅｔ ａｌ．: '"Ｐｒｏｄｕｃｔｉｏｎ ｏｆ ｈｉｇｈｌｙ ｈｏｍｏｇｅｎｅｏｕｓ ａｎｄ ｓｔｒｕｃｔｕｒａｌｌｙ ｉｎｔａｃｔ ｒｅｃｏｍｂｉｎａｎｔ ｖｏｎ Ｗｉｌｌｅｂｒａｎｄ ｆａｃｔｏｒ ｍｕｌｔｉｍｅｒ' ＢＩＯＴＥＣＨＮＯＬ． ＡＰＰＬ． ＢＩＯＣＨＥＭ． Ｖｏｌ．２４， Ｎｏ．３, 199612, ｐｐ．２５７-２６７
JPN6014018164; ＦＵＲＬＡＮ， Ｍ． ｅｔ ａｌ．: '"ｖｏｎ Ｗｉｌｌｅｂｒａｎｄ ｆａｃｔｏｒ-ｃｌｅａｖｉｎｇ ｐｒｏｔｅａｓｅ ｉｎ ｔｈｒｏｍｂｏｔｉｃ ｔｈｒｏｍｂｏｃｙｔｏｐｅｎｉｃ ｐｕｒｐｕｒａ ａｎｄ ｔｈｅ ｈｅｍｏｌｙｔｉｃ-ｕｒ' ＴＨＥ ＮＥＷ ＥＮＧＬＡＮＤ ＪＯＵＲＮＡＬ ＯＦ ＭＥＤＩＣＩＮＥ Ｖｏｌ．３３９， Ｎｏ．２２, 19981126, Ｐ．１５７８-１５８４
JPN6014018162; 日裏久英: '「ＴＭＡの診断と血小板輸血の指標に重要なＡＤＡＭＴＳ１３活性の新規測定法の開発」' 臨床病理 Ｖｏｌ．５５， Ｎｏ．１, 200701, Ｐ．６９-７５
JPN6015008544; ＴＳＡＩ， Ｈ．Ｍ． ｅｔ ａｌ．: '"Ｐｈｙｓｉｏｌｏｇｉｃ ｃｌｅａｖａｇｅ ｏｆ ｖｏｎ Ｗｉｌｌｅｂｒａｎｄ ｆａｃｔｏｒ ｂｙ ａ ｐｌａｓｍａ ｐｒｏｔｅａｓｅ ｉｓ ｄｅｐｅｎｄｅｎｔ ｏｎ ｉｔｓ ｃｏｎｆｏｒｍａｔｉｏｎ' ＢＬＯＯＤ Ｖｏｌ．８７， Ｎｏ．１０, 19960515, Ｐ．４２３５-４２４４
JPN6015037768; ＦＩＳＣＨＥＲ， Ｂ．Ｅ． ｅｔ ａｌ．: '"Ｓｔｒｕｃｔｕｒａｌ ａｎａｌｙｓｉｓ ｏｆ ｒｅｃｏｍｂｉｎａｎｔ ｖｏｎ Ｗｉｌｌｅｂｒａｎｄ ｆａｃｔｏｒ ｐｒｏｄｕｃｅｄ ａｔ ｉｎｄｕｓｔｒｉａｌ ｓｃａｌｅ ｆｅｒｍｅｎｔａｔｉｏｎ' ＦＥＢＳ ＬＥＴＴ． Ｖｏｌ．３７５， Ｎｏ．３, 19951120, ｐｐ．２５９-２６２
JPN6015037770; ＳＣＨＬＯＫＡＴ， Ｕ． ｅｔ ａｌ．: '"Ｐｒｏｄｕｃｔｉｏｎ ｏｆ ｈｉｇｈｌｙ ｈｏｍｏｇｅｎｅｏｕｓ ａｎｄ ｓｔｒｕｃｔｕｒａｌｌｙ ｉｎｔａｃｔ ｒｅｃｏｍｂｉｎａｎｔ ｖｏｎ Ｗｉｌｌｅｂｒａｎｄ ｆａｃｔｏｒ ｍｕｌｔｉｍｅｒ' ＢＩＯＴＥＣＨＮＯＬ． ＡＰＰＬ． ＢＩＯＣＨＥＭ． Ｖｏｌ．２４， Ｎｏ．３, 199612, ｐｐ．２５７-２６７
Attorney, Agent or Firm:
Hidesaku Yamamoto
Natsuki Morishita
Natsuki Morishita