Title:
代謝経路の生成または改変を可能とする進化した微生物の生産方法
Document Type and Number:
Japanese Patent JP2006517796
Kind Code:
A
Abstract:
Strain (A) of a microorganism which produces a 2-amino-4-alkylthio-butyric acid (I) by metabolizing a simple sugar and a thiol (II), or its salt, and has at least one gene encoding an enzyme with modified methionine synthase (MS) activity. Strain (A) of a microorganism which produces a 2-amino-4-alkylthio-butyric acid of formula (I) RS-(CH 2) 2-CHNH 2-COOH (I) by metabolizing a simple sugar and a thiol (II), R'-SH, or its salt, and has at least one gene encoding an enzyme with modified methionine synthase (MS) activity. R : 1-8C linear or branched alkyl, optionally substituted by one or more hydroxy or by (hetero)aryl with one or more nitrogen or sulfur atoms in the ring, i.e. phenyl, pyridyl, pyrroly, pyrazolyl, triazolyl, tetrazolyl, thiazolyl or thienyl; R' : hydrogen or as R. Independent claims are also included for the following: (1) modified cystathioninegamma -synthase (X) having MS activity; (2) nucleic acid (NA) that encodes (X); (3) cloning and/or expression vector containing (NA) and elements for regulating expression and transcription of (X); (4) method of screening for a bacterial strain that has a gene encoding (X) or an acylhomoserine sulfhydrylase (Y), for preparation of (A); and (5) method for producing (I) by culturing (A) or by using MS activity present in activated bacteria or their extracts.
Inventors:
Chateau michael
Gonzalez Benjamin
Mayneil-Sales Isabel
Soukary Philip Noel Paul
Zinc Olivia
Gonzalez Benjamin
Mayneil-Sales Isabel
Soukary Philip Noel Paul
Zinc Olivia
Application Number:
JP2006502150A
Publication Date:
August 03, 2006
Filing Date:
February 17, 2004
Export Citation:
Assignee:
Metabolic explorer
International Classes:
C12N15/09; C12N1/21; C12N9/10; C12N9/88; C12N15/01; C12N15/10; C12P11/00; C12P13/12
Attorney, Agent or Firm:
Hatsushi Shimizu
Koichi Niimi
Koichi Niimi