Methods of refining recombination ADAMTS13 and other protein, and those constituents

Japanese Patent JP6216930

B2

Provided herein are methods for purifying recombinant A Disintegrin-like and Metallopeptidase with Thrombospondin Type 1 Motif 13 (ADAMTS13) protein from a sample. The method comprises enriching for ADAMTS13 protein by chromatographically contacting the sample with hydroxyapatite under conditions that allow ADAMTS13 protein to appear in the eluate or supernatant from the hydroxylapatite. The methods may further comprise tandem chromatography with a mixed mode cation exchange/hydrophobic interaction resin that binds ADAMTS13 protein. Additional optional steps involve ultrafiltration/diafiltration, anion exchange chromatography, cation exchange chromatography, and viral inactivation. Also provided herein are methods for inactivating virus contaminants in protein samples, where the protein is immobilized on a support. Also provided herein are compositions of ADAMTS13 prepared according to said methods.

WO/2023/046886	FC BINDING POLYPEPTIDES
JPH0734859	Title of Invention Application of Hydrophobic Group Derivatized Poly (ethyleneimine) to Protein Immobilization for Immunoassay and Bioafinity Separation
JPH06510672	Description: Suppressor and progenitor cells

Mainhard Hass Lacquer
Artur Mitterer
Christian fiedler
Christa meyer

JP2015105808A

October 25, 2017

May 25, 2015

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Bax Alta Game Behr
Bax Alta Incorporated

C07K1/22; C07K14/745; C07K14/755; C07K16/24; C12N9/64

JP2003512594A
JP2005538686A
JP2007174977A
JP2003144154A

US20060246589
WO2009001743A1
WO2002031163A1

The Journal of Microbiology,2000年,Vol.38, No.3,p.187-191

Hidesaku Yamamoto
Natsuki Morishita
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto

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