Title:
ＣＲＩＳＰＲに基づくライブラリー調製およびシークエンシングのための方法およびシステム
Document Type and Number:
Japanese Patent JP7239988
Kind Code:
B2
Abstract:
Embodiments of a method and system for improved microbiome sequencing can include: generating guide RNA complexes for a set of targets corresponding to a set of taxa associated with the microorganism-related condition; processing the biological sample with the gRNA complexes to generate microorganism nucleic acid fragments comprising a set of end regions associated with the set of targets; ligating the set of end regions with a set of adapters sharing an adapter sequence; and amplifying the set of targets based on the ligated set of end regions and a set of primers sharing a primer sequence associated with the adapter sequence.
Inventors:
Apte, Zachary
Rich Man, Jessica
Armonasid, Daniel
Ortiz, Rodrigo
goddard, audrey
Bird, Sarah
Morales, Eduardo
Rich Man, Jessica
Armonasid, Daniel
Ortiz, Rodrigo
goddard, audrey
Bird, Sarah
Morales, Eduardo
Application Number:
JP2019516206A
Publication Date:
March 15, 2023
Filing Date:
September 27, 2017
Export Citation:
Assignee:
Somagen, Incorporated
International Classes:
C12Q1/6869; C12Q1/6806; C12Q1/686
Domestic Patent References:
| JP2016525355A |
Foreign References:
| WO2016100955A2 | ||||
| WO2016079731A2 | ||||
| US20140357523 |
Attorney, Agent or Firm:
Shusaku Yamamoto
Morishita Natsuki
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto
Morishita Natsuki
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto