Title:
アミノシランを用いるマイクロ及びナノコンタクトプリンティング：多重バイオアッセイのためのマイクロ流体デバイスのパターニング表面
Document Type and Number:
Japanese Patent JP6975466
Kind Code:
B2
Abstract:
It is an object of the present invention to achieve rapid surface patterning of biomolecules within microfluidic devices with high reproducibility. In this work, we present a new means of creating micro- and nano-patterns of aminosilanes within microfluidic devices via an aqueous based microcontact printing technique. To minimize the diffusion of molecules into the PDMS stamp, we use water as the inking solvent and enforce short incubation and contact times during the printing process to preserve the pre-defined resolution of patterned features. These patterns then serve as the building block to couple multiple biomolecules in solution onto a single surface for subsequent bioassays.
Inventors:
Amy Shen, Fried
Ricou, Sebastian Georg Gabriel
Ricou, Sebastian Georg Gabriel
Application Number:
JP2018558645A
Publication Date:
December 01, 2021
Filing Date:
February 01, 2017
Export Citation:
Assignee:
Okinawa Institute of Science and Technology Graduate School
International Classes:
G01N33/543; G01N33/547; G01N37/00
Domestic Patent References:
| JP2015531486A | ||||
| JP2009222401A |
Foreign References:
| WO2007123763A2 | ||||
| US20050079486 | ||||
| US20060263832 | ||||
| US20130157889 |
Other References:
FALCONNET, D. et al.,A novel approach to produce protein nanopatterns by combining nanoimprint lithography and molecular self-assembly,Nano Letters,2004年,Vol.4, No.10,p.1909-1914
GROLL, J. et al.,Biofunctionalized, Ultrathin Coatings of Cross-Linked Star-Shaped Poly(ethylene oxide) Allow Reversible Folding of Immobilized Proteins,JACS,2004年,Vol.126,p.4234-4239
ARKLES, B. et al.,Silane Coupling Agents,カタログ,Gelest, Inc.,2014年,p.27, p.54,https://www.gelest.com/wp-content/uploads/Silane_Coupling_Agents.pdf,[online], [令和3年6月8日検索], インターネット
GROLL, J. et al.,Biofunctionalized, Ultrathin Coatings of Cross-Linked Star-Shaped Poly(ethylene oxide) Allow Reversible Folding of Immobilized Proteins,JACS,2004年,Vol.126,p.4234-4239
ARKLES, B. et al.,Silane Coupling Agents,カタログ,Gelest, Inc.,2014年,p.27, p.54,https://www.gelest.com/wp-content/uploads/Silane_Coupling_Agents.pdf,[online], [令和3年6月8日検索], インターネット
Attorney, Agent or Firm:
Patent business corporation Tsukuni
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