Title:
スフィンゴイド塩基又はその誘導体を生産する微生物株
Document Type and Number:
Japanese Patent JP5156933
Kind Code:
B2
Abstract:
The present invention provides microbial strains, in particular yeast strains, that produce at least 0.1 mg per g biomass dry weight of a sphingoid base. The present invention further provides a method to obtain sphingoid base-producing microbial strains comprising incubating a population of microbial cells in the presence of a suitable concentration of a toxin, selecting cells that are resistant against said toxin, and isolating cells out of the toxin-resistant cell population that produce at least 0.1 mg per g biomass dry weight of the sphingoid base of Formula I. Optionally, the method further comprises subjecting a population of toxin-resistant microbial cells that produce at least 0.1 mg per g biomass dry weight of the sphingoid base of Formula I to DNA-mediated transformation with a polynucleotide encoding an enzyme of the sphingolipid metabolic pathway. The present invention further provides a polypeptide having dihydroceramide desaturase activity obtainable form Pichia ciferrii.
Inventors:
Van den berg, marco, alexander
Schaffel, Steffen
Schaffel, Steffen
Application Number:
JP2007539587A
Publication Date:
March 06, 2013
Filing Date:
November 07, 2005
Export Citation:
Assignee:
Evonik Industries AG
International Classes:
C12Q1/04; C12N1/19; C12N9/02; C12N15/09; C12P13/00; A61K31/133
Domestic Patent References:
| JP9504434A | ||||
| JP2002519070A | ||||
| JP2002513573A |
Other References:
Biochimica et Biophysica Acta,1973年,Vol.306,p.341-345
The Journal of Biological Chemistry,1998年,Vol.273, No.18,p.11062-11068
The Journal of Biological Chemistry,1998年,Vol.273, No.18,p.11062-11068
Attorney, Agent or Firm:
Yoshiyuki Inaba
Toshifumi Onuki
Toshifumi Onuki
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