PURPOSE: To enable easily, quickly and safely denaturating serum protein by processing serum sample before analyzing with microwave at the high dial.
CONSTITUTION: A serum sample to be analyzed is arranged in a microwave oven and exposed to microwave. The microwave oven has dials of low, middle and high and the high dial means that the magnetron emits microwave at 80 to 100% of the time, the oven is operated. For the microwave for example, one generated at 700W is used. The sample is processed at the high dial for approximately 20 to 90 seconds (60 seconds is most proper) processed by microwave. The serum sample is thus processed with microwave for at least 15 to 20 seconds and exposed in the temperature approximately 65 to 100°C (80 to 85°C is most proper). The serum sample is processed with microwave at this temperature for 60 seconds or less. In this manner, denaturation is caused in existing B12 or folate without disturbance as a whole.
JIERARUDAIN TAN ZAN
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