Title:
修飾された低分子干渉RNA分子および使用方法
Document Type and Number:
Japanese Patent JP4709545
Kind Code:
B2
Abstract:
The present invention provides double-stranded RNA molecules that mediate RNA interference in target cells, preferably hepatic cells. The invention also provides double-stranded RNA (dsRNA) molecules that are modified to be resistant to nuclease degradation, which inactivates a virus, and more specifically, hepatitis C virus (HCV). The invention also provides a method of using these modified RNA molecules to inactivate virus in mammalian cells and a method of making modified small interfering RNAs (siRNAs) using human Dicer. The invention provides modified RNA molecules that are modified to include a dsRNA or siRNA wherein one or more of the pyrimidines in the RNA molecule are modified to include 2′-Fluorine. The invention also provides dsRNA or siRNA in which all pyrimidines are modified to include a 2′-Fluorine. The invention provides that the 2′-Fluorine dsRNA or siRNA molecule is further modified to include a two base deoxynucleotide “TT” sequence at the 3′ end of the molecule.
Inventors:
Han Chan
Seomi Young
Houghton Michael
Seomi Young
Houghton Michael
Application Number:
JP2004524748A
Publication Date:
June 22, 2011
Filing Date:
July 25, 2003
Export Citation:
Assignee:
Novartis Bakushin's and Diagnostics, Incorporated
International Classes:
C12N15/09; A61K31/7105; A61K31/711; A61P31/12; A61P31/14; A61P31/16; A61P31/20; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/113; A61K38/00
Other References:
The Journal of Immunology,2002年11月,vol.169,p.5196-5201
LINDENBACH BRETT D,MOLECULAR CELL. UNITED STATES ,2002年 5月,V9 N5,P925-927
LINDENBACH BRETT D,MOLECULAR CELL. UNITED STATES ,2002年 5月,V9 N5,P925-927
Attorney, Agent or Firm:
Hatsushi Shimizu
Koichi Niimi
Koichi Niimi