Title:
MODIFIED NUCLEOTIDE
Document Type and Number:
Japanese Patent JP3170235
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To obtain a modified nucleotide comprising a specific modified nucleotide having detectable signal, capable of easily being detected when bonded to or enclosed in a nucleic acid material and useful as a probe, etc., for detection and isolation of DNA or RNA.
SOLUTION: This modified nucleotide is a new modified nucleotide of formulas I (P is a phosphate residue; S is residue of a saccharine or a monosaccharide; B is a residue of a base of purine or pyrimidine with the proviso that when the nucleotide is deoxyribonucleotide, the B binds with 3' and/or 5' position of phosphate residue, and when the nucleotide is ribonucleotide, the B binds with 2', 3' and/or 5' position of the phosphate residue; Sig is a chemical residue convalently bonding to a saccharide of a nucleotide, a monosaccharide residue S or a phosphate residue P, and allowing itself to be a signal, further, allowing itself to be detected or capable of informing its existence) or II. The modified nucleotide is useful as a recognizable probe, etc., used for detecting and isolating a DNA and a DNA sequence.
Inventors:
Dean Engel Heart
Elaza Ravani
Stanley Klein
Janice G. Stabriano Poulos
Dolly car taker
Elaza Ravani
Stanley Klein
Janice G. Stabriano Poulos
Dolly car taker
Application Number:
JP29588997A
Publication Date:
May 28, 2001
Filing Date:
October 28, 1997
Export Citation:
Assignee:
Enzo Biochem, Incorporated
International Classes:
A61K47/48; C07B59/00; C07H19/04; C07H19/10; C07H19/20; C12N9/00; C07H21/02; C07H21/04; C07K17/06; C08B37/00; C12N15/00; C12N15/09; C12Q1/68; C12Q1/70; G01N33/50; G01N33/53; G01N33/566; G01N33/58; (IPC1-7): C07H21/02; C12Q1/68; G01N33/50
Attorney, Agent or Firm:
Akira Asamura (1 person outside)
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