PURPOSE: To synthesize a monoclonal antibody or its biologically active fragment on the receptor for human tumor necrosis factor (hTNF-R) by selecting a hybridoma cell line secreting this antibody through a proper analytical system.
CONSTITUTION: A hybridoma cell strain secreting the antibodies is selected by means of a proper analytical system to synthesize a monoclonal antibody to the receptor for hTNF or its biologically active fragment. The monoclonal antibody or its biologically active fragment preferably has the activity of EC50 (the concentration of the Mabs where 5% TNF as % of the control is still bound to the tested cells) of down-modulation of 100 ng/ml or less on HL60 cells. The monoclonal antibody, when desired, non-toxic, inactive and therapeutically acceptable carrier are used to prepare its pharmaceutical composition.
HANSURUEJI RECHIERU
J.BIOCHEM=1986
PROC.NATL.ACAD.SCI.USA=1987
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