Title:
N-TERMINAL DERIVATIZATION OF PROTEINS WITH POLYSACCHARIDES
Document Type and Number:
Japanese Patent JP2015145400
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide a method for producing an N-terminal derivative of protein reacting N-terminal of protein or peptide under control conditions to form N-terminal derivative.SOLUTION: The invention provides a method for producing a purified N-terminal derivative of a protein or peptide comprising the steps of: (i) reacting N-terminal amine residue of protein or peptide with a polysaccharide in an acidic aqueous solution to form an N-terminal derivative; and (ii) purifying the obtained N-terminal derivative in an aqueous solution of a pH higher than that in the step (i). The invention also provides N-terminal derivatives of protein or peptide obtainable by the method. The controlled conditions include use of acidic pH for the derivatization step and a higher pH for purification. The derivatives are useful for improvement of pharmacokinetics and pharmacodynamics of protein and peptide.
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Inventors:
JAIN SANJAY
PETER LAING
GREGORIADIS GREGORY
PETER LAING
GREGORIADIS GREGORY
Application Number:
JP2015075148A
Publication Date:
August 13, 2015
Filing Date:
April 01, 2015
Export Citation:
Assignee:
LIPOXEN TECHNOLOGIES LTD
International Classes:
C07K1/113; A61K38/00; A61K38/21; A61K38/24; A61K38/46; A61P3/04; A61P7/06; A61P11/00; A61P35/00; A61P37/06; C07K14/555; C07K14/575; C12N9/22; C12N9/40
Domestic Patent References:
| JP2003533537A | 2003-11-11 | |||
| JPH09506116A | 1997-06-17 | |||
| JPH0733793A | 1995-02-03 | |||
| JPH04502331A | 1992-04-23 | |||
| JP2003533537A | 2003-11-11 | |||
| JPH09506116A | 1997-06-17 | |||
| JPH0733793A | 1995-02-03 | |||
| JPH04502331A | 1992-04-23 |
Foreign References:
| WO2005016974A1 | 2005-02-24 | |||
| WO2005016973A1 | 2005-02-24 | |||
| WO2006016168A2 | 2006-02-16 | |||
| WO2005037320A2 | 2005-04-28 | |||
| WO2005092928A1 | 2005-10-06 | |||
| WO2005016974A1 | 2005-02-24 | |||
| WO2005016973A1 | 2005-02-24 | |||
| WO2006016168A2 | 2006-02-16 | |||
| WO2005037320A2 | 2005-04-28 | |||
| WO2005092928A1 | 2005-10-06 |
Other References:
JPN6011051325; Ｂｉｏｃｈｉｍ． Ｂｉｏｐｈｙｓ． Ａｃｔａ． ｖｏｌ．１６２２, 2003, ｐ．４２-９
JPN6011051329; Ｉｎｔ． Ｊ． Ｐｈａｒｍ． ｖｏｌ．３００, 2005, ｐ．１２５-３０
JPN6011051325; Ｂｉｏｃｈｉｍ． Ｂｉｏｐｈｙｓ． Ａｃｔａ． ｖｏｌ．１６２２, 2003, ｐ．４２-９
JPN6011051329; Ｉｎｔ． Ｊ． Ｐｈａｒｍ． ｖｏｌ．３００, 2005, ｐ．１２５-３０
JPN6011051329; Ｉｎｔ． Ｊ． Ｐｈａｒｍ． ｖｏｌ．３００, 2005, ｐ．１２５-３０
JPN6011051325; Ｂｉｏｃｈｉｍ． Ｂｉｏｐｈｙｓ． Ａｃｔａ． ｖｏｌ．１６２２, 2003, ｐ．４２-９
JPN6011051329; Ｉｎｔ． Ｊ． Ｐｈａｒｍ． ｖｏｌ．３００, 2005, ｐ．１２５-３０
Attorney, Agent or Firm:
Patent Business Corporation Saegusa International Patent Office
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