Title:
【発明の名称】活性型ヒト好中球走化因子ポリペプチドの製造方法
Document Type and Number:
Japanese Patent JP3053631
Kind Code:
B2
Abstract:
A simple and efficient method of preparing active recombinant human neutrophil chemotactic factor. The human neutrophil chemotactic factor (NCF) is solubilized from a transformant cell homogenate using urea or guanidine hydrochloride. The polypeptide is further purified from the solution using conventional protocols such as CM-Sepharose CL-6B, HW-55 column, etc.. By using this method, large amounts of active human NCF can be recovered and purified.
Inventors:
Masaaki Yamada
Ryuji Furuta
Junichi Yamagishi
Matsushima koji
Ryuji Furuta
Junichi Yamagishi
Matsushima koji
Application Number:
JP50916489A
Publication Date:
June 19, 2000
Filing Date:
August 29, 1989
Export Citation:
Assignee:
THE UNITED STATES OF AMERICA
International Classes:
C07K1/14; C07K1/22; C07K14/00; C07K14/52; C07K14/54; C12N15/12; C12N15/09; C12P21/00; C12P21/02; C12R1/19; (IPC1-7): C12P21/02; C07K1/14; C12N15/09
Domestic Patent References:
| JP2502825A |
Attorney, Agent or Firm:
Yoshinori Hosoda