PURPOSE: To suppress the growth of ice crystal and protect the tissue, etc., of an object to be kept to low temperature by adding a freezing point depressing substance and a gelling agent consisting of carrageenan and a water-soluble protein to the object and keeping the object at a low temperature in gelatinized state.
CONSTITUTION: (A) A gelling agent produced by cross-linking carrageenan and a water-soluble protein (e.g. gelatin and albumen) and (B) a freezing point depressing substance (preferably an emulsion produced by adding a surfactant to glycerol, propyl alcohol, etc.) are adjusted to have a pH close to or lower than the isoelectric point of the protein and added to the object to be kept to a low temperature (e.g. sperm or fertilized ovum) to stably keep the object at low temperature in gelatinized state.
KIJIMA TOMIE
MIYAZAWA SETSUKO
TAKEHARA SACHIKO
