Title:
PRODUCTION OF CHIMERA OXIDASE
Document Type and Number:
Japanese Patent JP2569434
Kind Code:
B2
Abstract:
PURPOSE: To obtain a chimera oxidase having enhanced activities by expressing a vector, containing a gene having a monoatomic oxygenating active region of a rat hepatic cytochrome P-450 to a reducing activity feeding active region of a reduced nicotinamide adenine dinucleotide phosphate (NADPH)-reductase in a yeast fungus.
CONSTITUTION: A yeast expressing plasmid, containing a chimera oxidase gene constructed by connecting a region required for the monoatomic oxygenating activities of a rat hepatic cytochrome P-450 gene to a region required to manifest the reducing power feeding ability of a rat cytochrome NADPH-cytochrome P-450 reductase gene and capable of expressing the oxidase is transduced into a yeast fungus [e.g. Saccharomyces cerevisiae AH22 (pAMP19)] to transform the yeast fungus. The resultant transformant is then cultured to afford the objective chimera oxidase having the monoatomic oxygenating activities of the cytochrome P-450 and the reducing power feeding ability possessed by the NADPH-cytochrome P-450 reductase and the enhanced monoatomic oxygenating activities.
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Inventors:
Yoshiyasu Yabuzaki
Yuko Murakami
Toshiyuki Sakaki
Megumi Shibata
Hideo Okawa
Yuko Murakami
Toshiyuki Sakaki
Megumi Shibata
Hideo Okawa
Application Number:
JP34712295A
Publication Date:
January 08, 1997
Filing Date:
December 15, 1995
Export Citation:
Assignee:
Director of Industrial Technology
International Classes:
C12N15/09; C12N1/19; C12N9/02; C12R1/865; (IPC1-7): C12N15/09; C12N9/02