Title:
【発明の名称】ボルデテラ・ブロンキセプチカ感染症生菌ワクチン用原株の製造法および生菌ワクチン
Document Type and Number:
Japanese Patent JP2537042
Kind Code:
B2
Abstract:
A process for the preparation of a mutant strain of B. bronchiseptica having chromosomal genetic markers carrying at least temperature-sensitive (grown at 42 DEG C.), nalidixic acid-resistant and heat-labile dermonecrotic toxin producing ability negative markers, suitable for the preparation of live attenuated AR vaccine, which comprises subjecting a strain of B. bronchiseptica to an induced mutation, isolating a mutant having the genetic markers of being at least temperature-sensitive (grown at 42 DEG C.), nalidixic acid-resistant and heat-labile dermonecrotic toxin negative, culturing the isolated strain of B. bronchiseptica on a Bordet-gengou agar plate containing nalidixic acid at 42 DEG C., thereafter picking up an organism morphologically resembling a phase-I organism, repeating the procedures of culturing and picking up, and converting the organisms to a heat-labile dermonecrotic toxin-producing ability negative phase-I organism having a complete capsular antigen.
Inventors:
KUME KATSUMI
NAKAI TOYOJI
NISHIZAWA HIROSHI
YOSHIKAWA TAKASHI
DANBARA HIROFUMI
NAKAI TOYOJI
NISHIZAWA HIROSHI
YOSHIKAWA TAKASHI
DANBARA HIROFUMI
Application Number:
JP28089086A
Publication Date:
September 25, 1996
Filing Date:
November 27, 1986
Export Citation:
Assignee:
KITASATO INST
International Classes:
A61K39/10; C12N1/20; C12N15/00; C12N15/09; C12R1/01; (IPC1-7): C12N1/20; A61K39/10
Attorney, Agent or Firm:
Kazunori Kobayashi