Title:
MONOCLONAL ANTIBODY AGAINST LACTOBACILLUS AND DETECTION OF LACTOBACILLUS USING THE SAME ANTIBODY
Document Type and Number:
Japanese Patent JP2532330
Kind Code:
B2
Abstract:
PURPOSE: To provide a new monoclonal antibody capable of specifically recognizing a Lactobacillus lactis such as Lactobacillus plantarum and, therefore, quickly, sensitively and specifically detecting this harmful Lactobacillus contained in beer.
CONSTITUTION: A monoclonal antibody, e.g. monoclonal antibody 55-1C3 (Bikoken stipulation No. 13163) obtained by immunizing a mammal with Lactobacillus plantarum belonging to Lactobacillus. Production of the antibody is carried out by mixing Lactobacillus plantarum with an adjuvant, immunizing a mammal such as BALB/c mouse, etc., therewith, then fusing a splenic cell of the mammal to a myeloma, selecting a strain producing the objective antibody from the resultant hybridoma, cloning it and subsequently proliferating it in the abdominal dropsy of a mouse.
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Inventors:
NISHINAKA NAOKO
OOTAKE YASUYUKI
MORI TOSHIO
OOTAKE YASUYUKI
MORI TOSHIO
Application Number:
JP27928892A
Publication Date:
September 11, 1996
Filing Date:
September 25, 1992
Export Citation:
Assignee:
ASAHI BREWERIES LTD
International Classes:
C12N15/02; C07K16/12; C12N5/10; C12P21/08; G01N33/569; G01N33/577; C12N15/06; C12R1/91; (IPC1-7): C07K16/12; C12N5/10; C12N15/02; G01N33/577
Domestic Patent References:
| JP6210100A |
Other References:
ＢＵＬＬ．ＢＲＥＷ．ＳＣＩ．，２５（１９７９）Ｐ．２３−２８
Attorney, Agent or Firm:
Funakashi Eiko