Title:
ENZYME RELATED TO FORMATION OF AMIDE GROUP AT C-TERMINAL AND PRODUCTION AND USE THEREOF
Document Type and Number:
Japanese Patent JP2551399
Kind Code:
B2
Abstract:
PURPOSE: To provide a novel enzyme useful for forming a C-terminal amide group in a physiologically active peptide (calcitonin or secretin) in vitro by the formation of the amide group at the C-terminal.
CONSTITUTION: An enzyme relating to the formation of an amide group at the C-terminal of a C-terminated glycine adduct acts on a C-terminal α- hydroxyglycine adduct of formula I (A is a residual group excluding an α-amino group or imino group and an α-carboxyl group and originated from a natural α-amino acid; X is H, an amino acid derivative residual group bound to the N atom through a carbonyl group) to produce a terminal amide compound of formula II and a glyoxylic acid, has a mol. wt. (by a gel filtration method) of approximately 40000 dalton, an optimal pH of approximately 5-6, a stable pH of 4-9, and a suitable action temperature of 15-35°C. The enzyme can be prepared by treating an enzymatically active substance-containing material such as bovine serum with a substrate-affinitive chromatography having a C- terminal glycine adduct formula III as a ligand and subsequently with an anion exchange chromatography.
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Inventors:
IIDA TOSHIMOCHI
KAMINUMA TOSHIHIKO
FUSE YUKA
TAJIMA MASAHIRO
YANAGI MITSUO
OKAMOTO HIROSHI
KAMINUMA TOSHIHIKO
FUSE YUKA
TAJIMA MASAHIRO
YANAGI MITSUO
OKAMOTO HIROSHI
Application Number:
JP23915394A
Publication Date:
November 06, 1996
Filing Date:
October 03, 1994
Export Citation:
Assignee:
SHISEIDO CO LTD
International Classes:
C12N9/02; C12N9/00; C12P21/02; C12Q1/25; C12Q1/26; (IPC1-7): C12N9/00; C12P21/02; C12Q1/25
Attorney, Agent or Firm:
Takashi Ishida (3 others)