CORRECTING METHOD FOR SPECTRUM OF FLUORESCENCE SPECTROPHOTOMETER AND FLUORESCENCE SPECTROPHOTOMETER WITH SPECTRUM CORRECTING FUNCTION

Title:

CORRECTING METHOD FOR SPECTRUM OF FLUORESCENCE SPECTROPHOTOMETER AND FLUORESCENCE SPECTROPHOTOMETER WITH SPECTRUM CORRECTING FUNCTION

Document Type and Number:

Japanese Patent JPH0572039

Kind Code:

A

Abstract:

PURPOSE:To make it possible to perform highly accurate spectrum correction in a broad measuring range by utilizing an existing reference light source or sub-reference light source used for the wavelength-characteristic measurement on the fluorescence side, and obtaining the wavelength characteristic on the exciting side. CONSTITUTION:An optical quantum meter wherein rhodamine B is contained is set at the position of a sample cell 7. A fluorescence wavelength is set, and a spectroscope 2 on the exciting side undergoes wavelength scanning. The exciting-side wavelength characteristic in a wavelength region lambda1 (e.g. 200-600nm) is measured. The light from a sub-reference light source is made incidention a fluorescence-side spectroscope 9. The fluorescence-side wavelength characteristic in a wavelength region lambda2 (e.g. 500-900nm) is measured. Then, a diffusing element is set at the position of the cell 7. Both wavelengths on the exciting side and the fluorescence side are scanned at the same time for the whole wavelength region (200-900nm). The spectrum value, which is the product of both wavelengths is measured. The spectrum value and both wavelength characteristics are utilized, and the spectrum correcting functions for the fluorescence-side wavelength characteristic in the region lambda1, the exciting-side wavelength characteristic in the region lambda2 and the whole wavelength region are obtained. The functions are used for the true spectrum measurement and computation of an unknown sample.

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Inventors:

KOSHI HIROYUKI
OWADA MINORU

Application Number:

JP23470591A

Publication Date:

March 23, 1993

Filing Date:

September 13, 1991

Export Citation:

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Assignee:

HITACHI LTD

International Classes:

G01J3/443; G01N21/64; (IPC1-7): G01J3/443; G01N21/64

Attorney, Agent or Firm:

Akio Takahashi (1 person outside)

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