Title:
PRODUCTION OF POLYPEPTIDE
Document Type and Number:
Japanese Patent JP2515488
Kind Code:
B2
Abstract:
PURPOSE: To obtain a polypeptide such as chloramphenicol acetyl transferase and the like in high yield in high productivity by transforming a host with a specific expression vector, culturing the transformed host, and separating the polypeptide from the culture mixture.
CONSTITUTION: A DNA encoding a polypeptide of prokaryotic or eukaryotic cells effectively binds to and is kept by an expression-controlling DNA sequence that has DNA sequence of the formula and is formed by linking an Escherichia coli phage T5 promoter to an Escherichia coli lac operator or its functional moiety to prepare an expression vector, namely a plasmid that can be replicated in Gram-positive and/or Gram-negative bacterial cells. The host is transformed with the plasmid vector, the transformed strain is cultured under proper conditions, the polypeptide is isolated from the culture mixture whereby the objective polypeptide is obtained.
Inventors:
HERUMAN BUYARUDO
DEIETORITSUHI SUTEYUUBAA
DEIETORITSUHI SUTEYUUBAA
Application Number:
JP9517494A
Publication Date:
July 10, 1996
Filing Date:
May 09, 1994
Export Citation:
Assignee:
HOFFMANN LA ROCHE
International Classes:
C12N1/20; C12N1/21; C12N9/10; C12N15/00; C12N15/70; C12N15/72; C12P21/02; C12N15/09; C12R1/125; C12R1/19; (IPC1-7): C12P21/02; C12N9/10; C12N15/09
Domestic Patent References:
| JP57194790A |
Attorney, Agent or Firm:
Akira Asamura (2 outside)