Title:
NEW GENE, NEW YEAST WITH THE SAME SELECTIVELY DESTROYED AND USE OF THE YEAST
Document Type and Number:
Japanese Patent JP3373017
Kind Code:
B2
Abstract:
PURPOSE: To provide a new gene useful for producing thermosensitive autolytic yeast advantageous for acquiring yeast extract.
CONSTITUTION: The objective gene NLT1 having a base sequence coding protein having an amino acid sequence of the formula. Saccharomyces cerevisiae (FERM P-13410, hereafter referred to as YTS-1 strain), a thermosensitive autolytic mutant, is incorporated with a gene library constituted by incorporating plasmid YEp24 with the chromosome DNA of Saccharomyces cerevisiae X-2180 strain as a wild-type strain, and a YPD medium is inoculated with the transformant followed by culture at 37°C; plasmid pSLY-1 is then extracted from the resultant raised colonies followed by subcloning and then cutting with restriction enzymes Kpn1 and Cla2 to obtain the objective gene NLT1, a fragment of 3Kbp. A Saccharomyces yeast is incorporated with a plasmid destroying the gene NLT1 in a genetic engineering way in yeast to obtain the other objective Saccharomyces cerevisiae DLT11-1B strain (FERM P-13930) or the like, a thermosensitive autolytic yeast.
Inventors:
Tomoo Ogata
Yasushi Okumura
Yasushi Okumura
Application Number:
JP30475693A
Publication Date:
February 04, 2003
Filing Date:
November 10, 1993
Export Citation:
Assignee:
Asahi Breweries, Ltd.
International Classes:
A23L1/28; C07K14/37; C07K14/395; C07K14/41; C12N1/19; C12N15/09; C12P21/02; C12R1/865; (IPC1-7): C12N15/09; A23L1/28; C07K14/395; C12N1/19; C12P21/02
Attorney, Agent or Firm:
Eiji Tomomatsu (1 person outside)