NEW PHOSPHOLIPASE AND DNA CODING THE SAME

Japanese Patent JP3785602

B2

PROBLEM TO BE SOLVED: To obtain a new phospholipase bearing both phospholipase B activity and lipase activity, having a specific amino acid sequence or its variant, thus useful as e.g. a therapeutic agent for replenishing pancreatic phospholipase, a reagent for assaying phospholipase B/lipase. SOLUTION: This new phospholipase bears both phospholipase B activity and lipase activity, has the amino acid sequence at least from the Lys at 367 site to the Asn at 712 site in an amino acid sequence of the formula, or an amino acid sequence modified by substitution, deletion or addition of one or several amino acids in the above sequence, and is useful as e.g. a therapeutic agent for replenishing pancreatic phospholipase, a reagent for assaying phospholipase B/lipase. This new phospholipase is obtained by purification with both column chromatography and electrophoresis of a component limitedly decomposed during preserving a rat small intestine at -35°C for about 2 months after excised under its pentobarbital anesthetization and then solubilized from the brush border membrane.