Title:
NEW PROTEASE, ITS PRODUCTION AND NEW GENUS ALTEROMONAS MICROORGANISM
Document Type and Number:
Japanese Patent JP3609102
Kind Code:
B2
Abstract:
PURPOSE: To provide the new protease having specific physicochemical properties and having a Mytilus edulis Linne byssus-decomposing ability.
CONSTITUTION: The objective protease has e.g. following physicochemical properties; action: has a Mytilus edulis Linne byssus-decomposing ability; substrate specificity: cleaves the bond between the third Asn and the fourth Gln, and the bond between the fourth Gln and the fifth His in a formic peracid-oxidized bovine insulin B chain of the sequence No.1 in the sequence table, and further cleaves the bond between the eighth Thr and the ninth L-dopa, and the bond between the ninth L-dopa and the tenth Lys in the decapeptide of the sequence No.2; optimal pH: 9.5-10; optimal temperature: 50-55°C; optimal NaCl concentration: 5%; mol.wt.: 44000; inhibitor: the enzyme is inhibited with phenylmethanesulfonyl fluoride. The protease is obtained by culturing Alteromonas.peptidysis and separating the protease from the cultured product containing the produced protease.
Inventors:
Nobuhiko Domoto
Casturi Venkat Swallan
Hiromi Tanaka
Executive Wataru
Casturi Venkat Swallan
Hiromi Tanaka
Executive Wataru
Application Number:
JP7500093A
Publication Date:
January 12, 2005
Filing Date:
March 10, 1993
Export Citation:
Assignee:
Marine Biotechnology Research Institute Co., Ltd.
International Classes:
A01N37/44; C02F1/00; C02F3/34; C12N1/20; C12N9/52; C12R1/01; (IPC1-7): C12N9/52; C02F1/00; C02F3/34; C12N1/20
Other References:
平成3年度日本水産学会秋季大会学会要旨集,1991年10月 7日,第128頁546
Abstract of International Marine Biotechnology Conference,1991年10月,p.82
Abstract of International Marine Biotechnology Conference,1991年10月,p.82
Attorney, Agent or Firm:
Yusuke Hiraki
Ishii Teiji
Yasushi Hayakawa
Ishii Teiji
Yasushi Hayakawa