新規ルーメンバクテリア変異菌株及びそれを用いたコハク酸の製造方法

Japanese Patent JP4672671

B2

Provided are novel rumen bacterial mutants resulted from the disruption of a lactate dehydrogenase gene (ldhA) and a pyruvate formate-lyase gene (pfl) from rumen bacteria; a novel bacterial mutant (Mannheimia sp. LPK7) having disruptions of a ldhA, a pfl, a phosphotransacetylase gene (pta), and a acetate kinase gene (ackA); a novel bacterial mutant (Mannheimia sp. LPK4) having disruptions of a ldhA, a pfl, and a phosphoenolpyruvate carboxylase gene (ppc) involved in the immobilization of CO2 in a metabolic pathway of producing succinic acid; and a method for producing succinic acid, characterized by culture of the above mutants in anaerobic conditions. The bacterial mutants have the property of producing succinic acid at high concentration while producing little or no organic acids, as compared to the prior wild-type strains of producing various organic acids. Thus, the bacterial mutants are useful as strains for the industrial production of succinic acid.

Issanyup
Issanjun

JP2006541014A

April 20, 2011

May 20, 2004

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ELECTRONICS AND TELECOMMUNICATIONS RESEARCH INSTITUTE

C12N15/09; C12N1/20; C12N1/21; C12P7/44; C12P7/46; C12R1/01

JP2000500333A

WO2002000846A1

Appl. Microbiol. Biotechnol.,2002年,vol.58,pp.663-668
Database GenBank, [online],2010年 3月 5日,アクセッション番号AE016827,[平成22年3月12日検索],URL,http://www.ncbi.nlm.nih.gov/nuccore/52306107
Database GenBank, [online],2004年 9月17日,Revision history for AE016827,[平成22年3月12日検索],URL,http://www.ncbi.nlm.nih.gov/sviewer/girevhist.cgi?val=AE016827&log$=seqview
APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2006年,vol.72 no.3,pp.1939-1948

Kenichi Morita

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