Title:
新規なsiRNA遺伝子ライブラリー、その製造方法及び使用
Document Type and Number:
Japanese Patent JP2005533503
Kind Code:
A
Abstract:
The present invention relates to methods and compositions for the elucidation of gene function and the identification of novel genes. Specifically, the present invention relates to methods and compositions for improved functional genomic screening, functional inactivation of specific essential or non-essential genes, and identification of genes that are modulated in response to specific stimuli or encode recognizable phenotypic traits. In particular, the compositions of the present invention include, but are not limited to, expression cassettes comprising a novel dual promoter transcription system, that utilizes modified promoters, preferably containing complementary termination sequences, positioned across a coding sequence and in opposite orientation to each other. In addition, the present invention includes libraries comprising the expression cassettes of the invention, including vectors for transforming cells, such as replication-deficient retroviral vectors. The invention also includes methods for the production and screening of dsRNA/siRNA libraries, as well as therapeutic uses for the siRNAs expressed in accordance with the invention.
Inventors:
Lee Henry
Chatterton John Yee
Kenin
Wonstal Flossy
Chatterton John Yee
Kenin
Wonstal Flossy
Application Number:
JP2004523382A
Publication Date:
November 10, 2005
Filing Date:
July 23, 2003
Export Citation:
Assignee:
IMUSOL Inc.
International Classes:
C12N15/09; C12N15/10; C12N15/11; (IPC1-7): C12N15/09
Attorney, Agent or Firm:
Sadao Kumakura
Nobuo Ogawa
Atsushi Hakoda
Kenji Asai
Koji Hirayama
Nobuo Ogawa
Atsushi Hakoda
Kenji Asai
Koji Hirayama