PURPOSE: To obtain the subject novel compound comprising a 19-membered ring macrolactone and having an antifungal activity and an antitumor activity by culturing a microorganism belonging to the genus Amycolatopsis in a medium, and subsequently extracting the cultured product from the culture solution.
CONSTITUTION: A microorganism [e.g. Amycolatopsis sp. TA-0234 (FERM P-13322)] belonging to the genus Amycolatopsis is inoculated into a sterile liquid medium, and subsequently cultured at 28°C for 96hr under shaking. The culture solution is added to a medium in a mini jar, cultured at 28°C for 96hr under stirring and aeration bubbles, and subsequently extracted with acetone. The acetone is distilled off from the extract solution, and the residue is extracted with ethyl acetate. The extract solution is concentrated into a brown syrup-like substance. The substance is dissolved in methanol and subjected to a gel filtration. An separated active fraction is passed through a reverse phase silica gel column, and an adsorbed substance is eluted and subsequently purified with a high performance liquid column chromatography to provide the objective novel 19-membered ring macrolactone having an antifungal activity and an antitumor activity.
AKASHI SATOSHI
MIZOGAMI KAZUTOSHI
NAGATE TAKATOSHI
HANADA KAZUNORI
