PURPOSE: To prepare the titled antibiotic substance having antibacterial activity against Gram-negative and Gram-positive bacteria, by culturing a specific microbial strain belonging to Dactylosporangium genus and capable of producing the antibiotic substance, and separating from the cultured product.
CONSTITUTION: Microbial strain belonging to Dactylosporagium genus and capable of producing the antibiotic substance SF-2107A2 is cultured, and the objective antibiotic substance SF-2107A2 is separated from the cultured product. Glucose, starch, molasses, soybean oil, etc. can be used as the nutrient source for the cultivation. The nitrogen source is, e.g. soybean flour, wheat embryo, meat extract, dried yeast, fish meal, ammonium sulfate, urea, etc. The submerged method is the most suitable cultivation method, and the temperature is preferably 25W32°C. The concentration of SF-2107A2 substance reaches its peak in 3W10 days by either shake cultivation or tank cultivation.
SHIYOUMURA TAKASHI
AMANO SHIYOUICHI
NIWA TOMIZOU
KOJIMA MICHIO
ITOU TATSUO
