NOVEL ANTIBODY AND DETECTION OF HUMAN HEMOGLOBIN USING SAME

Title:

NOVEL ANTIBODY AND DETECTION OF HUMAN HEMOGLOBIN USING SAME

Document Type and Number:

Japanese Patent JPS63273472

Kind Code:

A

Abstract:

NEW MATERIAL:Anti-human HbmAb capable of selectively bonding to human Hb and hybridoma cell-line.

USE: A reagent composed of anti-HbmAb and a test membrane containing said antibody is useful for the test of occult blood. The reagent enables accurate examination with simple operation without necessitating restriction in diet.

PREPARATION: A myeloma cell is fused with an antibody-producing cell produced by immunizing an animal other than human with human Hb as an antigen. The obtained hybridoma is cultured in a medium or transplanted into peritoneal cavity of mouse to form ascites and obtain anti-HbmAb capable of selectively bonding to human Hb. The antibody is supported on a membrane composed of a polymer containing introduced epoxy group, etc., to obtain an antibody-bonded membrane. A determination kit is prepared by combining said membrane with an indicator-containing membrane or using an antibody- bonded membrane containing color-developing agent.

Inventors:

IIJIMA NOBORU
YAMAMURA HATSUO
KITAHATA KATSUAKI

Application Number:

JP10704987A

Publication Date:

November 10, 1988

Filing Date:

April 30, 1987

Export Citation:

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Assignee:

ASAHI BREWERIES LTD

International Classes:

G01N33/53; C12N5/00; C12N5/10; C12N15/00; C12N15/02; C12P21/00; C12P21/08; G01N33/577; C12R1/91; (IPC1-7): C12N5/00; C12N15/00; C12P21/00; C12R1/91; G01N33/53; G01N33/577

Domestic Patent References:

JPS59182367A	1984-10-17
JPS61172064A	1986-08-02
JPS61280571A	1986-12-11

Attorney, Agent or Firm:

Morio Sada

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