NEW MATERIAL:Compounds of formula I: (R1 is H or lower alkyl group; R2 is H or amino protective group; R3 is antigenic molecule amino residue; antigenic molecule amino residue having terminal amino group).
EXAMPLE: Aziryl-glycyl-insulin.
USE: Intermediates for compounds usable in Enzymo-immuno assay, scarcely deteriorating the enzymic and immunological activites. No side reactions.
PROCESS: Aziryl carboxylic acid derivatives of formula II: (R2' is amino protective group; R4 is active ester) are reacted with antigenic molecules corresponding to R3, antigenic molecules, e.g. primary antibody, hapten, etc., or antigenic molecules having terminal amino groups, and, if necessary, with removal of R2' to give the objective compounds of formula I. The enzymo-immuno assay compounds can be obtained by reacting compounds of formula I, wherein R2 is H, with an enzyme
YAMAUCHI HIROYO
KITAZAWA SHIYUUICHI
NODA TOSHIHARU
OOKAWA KENJI
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