Title:
新規エンテロキナーゼ開裂配列
Document Type and Number:
Japanese Patent JP2004503219
Kind Code:
A
Abstract:
Novel enterokinase cleavage sequences are provided. Also disclosed are methods for the rapid isolation of a protein of interest present in a fusion protein construct including a novel enterokinase cleavage sequence of the present invention and a ligand recognition sequence for capturing the fusion construct on a solid substrate. Preferred embodiments of the present invention show rates of cleavage up to thirty times that of the known enterokinase cleavage substrate (Asp)4-Lys-Ile.
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Inventors:
Lee, arthur, charles
Lunueu, christopher, john
Radner, Robert, Charles
Lunueu, christopher, john
Radner, Robert, Charles
Application Number:
JP2002504321A
Publication Date:
February 05, 2004
Filing Date:
June 19, 2001
Export Citation:
Assignee:
Diax corporation
International Classes:
G01N33/53; C07K7/06; C07K14/00; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/50; C12N15/09; C12P21/02; C12Q1/02; C12Q1/48; C12Q1/68; G01N33/566; (IPC1-7): C12N15/09; C07K14/00; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/50; C12P21/02; C12Q1/02; C12Q1/48; C12Q1/68; G01N33/53; G01N33/566
Domestic Patent References:
| JPH06113878A | 1994-04-26 |
Foreign References:
| WO1993003157A1 | 1993-02-18 |
Attorney, Agent or Firm:
Yusuke Hiraki
Satoshi Fujita
Natsuo Tanaka
Satoshi Fujita
Natsuo Tanaka