Title:
NUCLEIC ACID AMPLIFICATION METHOD, METHOD FOR PRODUCING NUCLEIC ACID SAMPLE FOR NUCLEIC ACID AMPLIFICATION AND NUCLEIC ACID ANALYZING METHOD
Document Type and Number:
Japanese Patent JP2015096061
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide a nucleic acid amplification method and a method for producing a nucleic acid sample for nucleic acid amplification which can collect nucleic acids simply and efficiently in a short time, without requiring enzyme treatment for tissue lysis in extracting nucleic acids from a biological tissue.SOLUTION: The method of the invention is characterized by comprising a heat treatment step of heating a mixture of an isolated biological tissue and an aqueous solvent, and releasing nucleic acids from the biological tissue; and an amplification step of performing nucleic acid amplification in the reaction solution comprising a nucleic acid sample, using the mixture after the heat treatment step as the nucleic acid sample, and using nucleic acids in the nucleic acid sample as a template. In the heat treatment step, the heat treatment time is 5 to 60 minutes. In the amplification step, the nucleic acid sample comprised in the reaction solution per 50 μL is obtained from 0.01-1 mmof the total volume of the biological tissue. In before and after the heat treatment step, enzyme treatment by an enzyme for tissue lysis is not performed.
Inventors:
ITO MASAFUMI
GUNJI MASAHARU
SUGIYAMA CHISAKI
MURAKAMI KAHO
SUZUKI SHUNICHI
GUNJI MASAHARU
SUGIYAMA CHISAKI
MURAKAMI KAHO
SUZUKI SHUNICHI
Application Number:
JP2014206962A
Publication Date:
May 21, 2015
Filing Date:
October 08, 2014
Export Citation:
Assignee:
ARKRAY INC
ITO MASAFUMI
GUNJI MASAHARU
ITO MASAFUMI
GUNJI MASAHARU
International Classes:
C12N15/09; C12Q1/68
Other References:
JPN7016001127; Ｊｏｕｒｎａｌ ｏｆ Ｃｌｉｎｉｃａｌ Ｐａｔｈｏｌｏｇｙ Ｖｏｌ．４７, 1994, ｐ．３１８-３２３
JPN7016001128; Ｊｏｕｒｎａｌ ｏｆ Ｃｌｉｎｉｃａｌ Ｐａｔｈｏｌｏｇｙ： Ｍｏｌｅｃｕｌａｒ Ｐａｔｈｏｌｏｇｙ Ｖｏｌ．５３, 2000, ｐ．５１-５２
JPN6016017014; ＷＡＫＯ ＢＩＯ ＷＩＮＤＯＷ Ｎｏ．２５, 2000, ｐ．６
JPN6016017017; ＴａＫａＲａ ＤＥＸＰＡＴ（ＤＮＡ Ｅｘｔｒａｃｔｉｏｎ ｆｒｏｍ Ｐａｒａｆｆｉｎ-ｅｍｂｅｄｄｅｄ Ｔｉｓｓｕｅ） 説明書 , 201209, ｐ．１-１２
JPN6016017020; ＵＰＬＯＡＤ ＴＯＹＯＢＯ ＢＩＯＣＨＥＭＩＣＡＬＳ Ｖｏｌ．９９, 2012, ｐ．８
JPN7016001128; Ｊｏｕｒｎａｌ ｏｆ Ｃｌｉｎｉｃａｌ Ｐａｔｈｏｌｏｇｙ： Ｍｏｌｅｃｕｌａｒ Ｐａｔｈｏｌｏｇｙ Ｖｏｌ．５３, 2000, ｐ．５１-５２
JPN6016017014; ＷＡＫＯ ＢＩＯ ＷＩＮＤＯＷ Ｎｏ．２５, 2000, ｐ．６
JPN6016017017; ＴａＫａＲａ ＤＥＸＰＡＴ（ＤＮＡ Ｅｘｔｒａｃｔｉｏｎ ｆｒｏｍ Ｐａｒａｆｆｉｎ-ｅｍｂｅｄｄｅｄ Ｔｉｓｓｕｅ） 説明書 , 201209, ｐ．１-１２
JPN6016017020; ＵＰＬＯＡＤ ＴＯＹＯＢＯ ＢＩＯＣＨＥＭＩＣＡＬＳ Ｖｏｌ．９９, 2012, ｐ．８
Attorney, Agent or Firm:
Koichiro Tsujimaru
Yumi Nakayama
Isaji So
Yumi Nakayama
Isaji So