リガンドを高感度に検出する核酸分子並びに該核酸分子のスクリーニング方法および該核酸分子の感度の最適化方法

Japanese Patent JP5731016

B2

It is an object of the present invention to provide nucleic acid molecules that enable highly sensitive detection of ligands (e.g., patulin). It is another object of the present invention to provide a screening method for nucleic acid molecules that enable highly sensitive detection of ligands (e.g., patulin), and a method for screening for nucleic acid molecules used for the optimization of nucleic acid molecules that enable highly sensitive detection of ligands (e.g., patulin). It is a further object of the present invention to provide a method for effectively removing ligands from samples containing ligands (e.g., patulin). According to the present invention, there is provided a loop-structured nucleic acid molecule for detection of ligands (e.g., patulin) having a DNA aptamer and a DNAzyme, wherein the sequence is modified between the DNA aptamer region and the DNAzyme.

Yasuyuki Tomita
Yuji Morita
Daisuke Fujiwara

JP2013552441A

June 10, 2015

April 25, 2013

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Kirin Company Limited

C12N15/09; C12M1/00; C12N15/115; C12Q1/68; G01N37/00

JP2010158238A	2010-07-22
JP2009520956A	2009-05-28
JP2002528109A	2002-09-03
JP2008515405A	2008-05-15

WO2012002541A1

2012-01-05

JPN6014013828; Ａｎａｌ　Ｃｈｅｍ．，　２００９年，　第８１巻，　９１１４-９１１９ページ
JPN6014013831; Ａｎａｌ　Ｃｈｅｍ．，　２０１０年，　第８２巻，　４３９６-４４０２ページ
JPN6014013833; 岩谷直治記念財団研究報告書，　２００４年，　第２７巻，　３６-３８ページ
JPN6014029937; Ｂｉｏｓｅｎｓ　Ｂｉｏｅｌｅｃｔｒｏｎ．，　２０１２年２月，　第３２巻，　２０８-２１２ページ
JPN6014029940; Ｊ　Ｎｕｃｌｅｉｃ　Ａｃｉｄｓ．，　２０１２年１０月，　２０１２：７４８９１３

Noritaka Yokota

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