Title:
NUCLEIC ACID PROBE-IMMOBILIZED SUBSTRATE AND METHOD FOR DETECTING THE PRESENCE OF TARGET NUCLEIC ACID BY USING THE SAME
Document Type and Number:
Japanese Patent JP3917640
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To provide a probe-immobilized substrate that enables high-efficiency hybridization.
SOLUTION: The method for detecting the presence of target nucleic acid comprises following three steps: the step where the primer satisfying the X, Y relation of X≥Y, Y≥10 &angst and 200 &angst ≥X is used to amplify the sample nucleic acid of more than 70 bases in the total length; the step where the amplified product obtained from the above-stated amplification is allowed to react with the nucleic probe; and the step where the presence of the target nucleic acid is judged in the nucleic acid sample by detecting the hybridization caused by the pre-step reaction.
More Like This:
Inventors:
Tadashi Takahashi
Jun Okada
Koji Hashimoto
Jun Okada
Koji Hashimoto
Application Number:
JP2006224283A
Publication Date:
May 23, 2007
Filing Date:
August 21, 2006
Export Citation:
Assignee:
Toshiba Corporation
International Classes:
C12Q1/68; C12N15/09
Domestic Patent References:
| JP3504328A | ||||
| JP5168499A | ||||
| JP5285000A | ||||
| JP6245800A | ||||
| JP10146183A | ||||
| JP62157570A | ||||
| JP10505492A | ||||
| JP9503307A |
Other References:
Am.Chem.Soc. Symp.Ser., No.728,pp.190-204 (1999)
Attorney, Agent or Firm:
Takehiko Suzue
Satoshi Kono
Makoto Nakamura
Kurata Masatoshi
Takashi Mine
Yoshihiro Fukuhara
Sadao Muramatsu
Ryo Hashimoto
Satoshi Kono
Makoto Nakamura
Kurata Masatoshi
Takashi Mine
Yoshihiro Fukuhara
Sadao Muramatsu
Ryo Hashimoto