Title:
NUCLEOTIDE REAGENT
Document Type and Number:
Japanese Patent JP3170241
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To obtain a new nucleic acid monomer comprising a specific polyfunctional nucleic acid monomer, and useful for contamination of a selective cleaving site and an abasic site to an oligonucleotide chain, and determination of the oligonucleotide, and further useful as a phosphorylation agent, etc.
SOLUTION: This nucleic acid monomer is a new polyfunctional nucleic acid monomer represented by formula I {R1 is a base-stable and acid-sensitive blocking group; R2 is a phosphoric acid derivative enabling a nucleic acid to be added to 5'-position of an oligonucleotide chain in the chemical synthesis; R3 is a halogen, methyl, I, Br or F; R4 is H car methyl; R5 is revulinyl, a group of formula II [Rk is an amino, nitro, a halogeno, OH, a lower alkyl or a lower alkoxy; (k) is 0-4], etc.; Z is a group of formula III [(x) is 1-8], etc.}. The nucleotide reagent is useful for contamination of a selective cleaving site and/or an abasic site to an oligonucleotide chain, a chemical assay such as determination of the oligonucleotide, a reagent for phosphorylation, etc.
Inventors:
Ardea, Michael Es.
Horn, thomas
Horn, thomas
Application Number:
JP8582098A
Publication Date:
May 28, 2001
Filing Date:
March 31, 1998
Export Citation:
Assignee:
Bayer Corporation
International Classes:
C07F9/10; C07F9/24; C07F9/40; C07F9/6512; C07F9/06; C07F9/655; C07H1/02; C07H11/04; C07H15/10; C07H15/18; C07H15/207; C07H19/06; C07H19/073; C07H19/10; C07H21/00; C07H21/04; C07H23/00; C12N15/09; C12Q1/68; G01N33/50; G01N33/52; (IPC1-7): C07H19/10; C07H21/04; C12Q1/68
Attorney, Agent or Firm:
Takashi Ishida (4 others)