Title:
ヌクレオチド配列のPCR増幅および検出方法および装置
Document Type and Number:
Japanese Patent JP4376188
Kind Code:
B2
Abstract:
A DNA-Chip includes a flat carrier and an array of spots containing probe molecules (oligonucleotides) which are arranged on said carrier. Each spot is associated with a microelectrode arrangement for impedance spectroscopic detection of binding events occurring between the probe molecules and target molecules (DNA fragments) applied by way of an analyte solution. In order to increase the sensitivity or the binding specific measuring effects of the biochip, the electrode arrangement is at least partially embedded in a hydrophilic reaction layer containing probe molecules and which is permeable to target molecules.
Inventors:
Gumbrecht, Walther
Stanzel, manfred
Stanzel, manfred
Application Number:
JP2004561040A
Publication Date:
December 02, 2009
Filing Date:
December 15, 2003
Export Citation:
Assignee:
Siemens Aktiengesellschaft
International Classes:
C12Q1/68; C12N15/09; G01N27/30; G01N27/416; G01N37/00; C12M1/00
Domestic Patent References:
| JP2001525921A |
Foreign References:
| WO2000062036A1 | ||||
| WO2000060919A1 | ||||
| US5736257 | ||||
| WO2002020833A1 | ||||
| WO2000058522A1 |
Other References:
Fuchs,A. et al.,"A silicon lab-on-chip for integrated sample preparation by PCR andDNA analysis by h,P227-231
Attorney, Agent or Firm:
Iwao Yamaguchi