Title:
【発明の名称】細胞培養でインターフェロン産生を高める方法
Document Type and Number:
Japanese Patent JP3432519
Kind Code:
B2
Abstract:
Methods for enhancing the production of viral vaccines in animal cell culture are described. These methods rely on the manipulation of the cellular levels of certain interferon induced antiviral activities, in particular, cellular levels of double-stranded RNA (dsRNA) dependent kinase (PKR) and 2'-5' oligoadenylate synthetase (2-5A synthetase). In cell cultures deficient for PKR or 2-5A synthetase, viral yield is enhanced by several orders of magnitude over cell cultures with normal levels of these proteins making these cell cultures useful for the production of viral vaccines.
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Inventors:
Lowe, Alan, S.
Application Number:
JP51045097A
Publication Date:
August 04, 2003
Filing Date:
August 22, 1996
Export Citation:
Assignee:
The Regents of the University of California
International Classes:
A61K39/12; A61K39/125; A61K39/145; A61K39/205; C12N15/09; C07K14/47; C07K14/555; C12N7/00; C12N7/01; C12N9/12; C12N15/113; C12N15/19; C12N15/67; C12P21/02; C12Q1/18; C12Q1/70; A61K38/00; C12R1/91; (IPC1-7): C12N15/09; C07K14/47; C07K14/555; C12P21/02
Attorney, Agent or Firm:
Atsushi Nakajima (2 outside)